複選題

27. Which of the following gene transcripts of RNA polymerase II in eukaryotic cells?
(A) 45S ribosomal RNA
(B) Glyceraldehyde dehydrogenase mRNA
(C)
(D) U6 snRNA
(E) β-actin mRNA

28. E. coli does not express β-galactosidase when its growth medium contains glucose, because (A) CAP is not activated for DNA binding to promote transcription initiation of β-Galactosidase gene. (B) Glucose stimulates the expression of lac repressor. (C) Lac repressor binds to the lac operator sequence. (D) Glucose interferes with lactose binding with the lac repressor. (E) Glucose binds to Lac operator

29. Which of the following restriction enzymes when used to completely digest an E. coli genomic DNA sample would generate DNA fragments in average size of 256 bp. (A) Not I: GC/GGCCGC (B) Hind III: A/AGCTT (C) Hpa II: C/CGG (D) Hpa I: GTT/AAC (E) Alul AG/CT

複選題30. Which of the following amino acids are in the zinc finger motif? (A) Tryptophan (B) Histidine (C) Cysteine (D) Tyrosine (E) Leucine

複選題31. Restrition enzymes cut DNA double helix to generate: (A) 5'-protruding ends (B) 3'-protruding ends (C) blunt ends (D) one 5'-protruding end and one blunt end (E) one 3'-protruding end and one blunt end

32. During mitosis, the nuclear membrane of a cell: (A) disappears temporarily (B) does not disappear (C) disappear permanently (D) disappears partially (E) disappears randomly

33. Mutations in a gene could generate (A) a polypeptide with amino acid changes (B) a polypeptide with no amino acid changes (C) a shorter peptide (D) a longer peptide (E) two shorter peptides

34. Eukaryotic translation of mRNAs is processed in (A) nucleus (B) mitochondria (C) cytosol (D) ER (E) Golgi

35. Which one of the following amino acids can form covalent bonds with other amino acids? (A) lysine (B) valine (C) cystein (D) alanine (E) proline

36. Which of the following is used as the substrate by DNA polymerase during DNA replication? (A) nucleotide diphosphate (B) nucleotide triphosphate (C) nucleotide monophosphate (D) nucleotide diphosphate and triphosphate (E) nucleotide monophosphate and triphosphate

37. Prior to cell fractionation, cells may be ruptured by placing them in (A) sonicator (B) high speed blender (C) an isotonic solution (D) an hypotonic solution (E) an hypertonic solution

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