複選題

35. Which of the following is   NOT   associated with eukaryotic translation initiation?
(A) Shine-Dalgarno sequence
(B) a formylated Met--tRNA met
(C) base pairing between the mRNA and 16S rRNA
(D) all of the above

36. During translation, translocation of the ribosome is driven by which of the following? (A) GTP-GDP + P (B) ATP ADP + P＜sub＞i＜/sub＞ (C) ATP AMP + PP＜sub＞i＜/sub＞ (D) Release of the tRNA in the P site

37. Which of the following is correct? (A) Transfer of genetic information via direct cell-cell contact is called transfection. (B) Specialized transduction can be carried out by any bacteriophage. (C) Transformation has been observed in only a limited number of bacteria in nature, but we have found ways to force the process upon other species in the laboratory. (D) All of above

38. Which set of forward and reverse primers will be appropriate to amplify the following gene? ATGGATCCAAACACTGTGTCAAGCTTTCAGGACATACTGCTGAGGATGTCAAAAATGCAGTT GGAGTCCTCATCGGGGGACTTGAATGGAATGATAACACAGTTCGAGTCTCTGAAACTCTACA GAGATTCGCTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACTCACTCCAAAACAGAAA CGAGAAATGG GGAACAATTAGGTCAGAAGTTTGAAGAAATAAGATGGTTGATTGAAGAA GTGAGACACAAACTGAAGATAACAGAGAATAGTTTTGAGCAAATAACATTTATGCAAGCCTT ACATCTATTGCTTGAAGTGGAGCAAGAGATAAGAACTTTCTCGTTTCAGCTTATTTAG (A) ATGGATCCAAACACTGTGTCAAGC; CTTTCTCGTTTCAGCTTATTTAG (B) ATGGATCCAAACACTGTGTCAAGC; CTAAATAAGCTGAAACGAGAAAG (C) GCTTGACACAGTGTTTGGATCCATT; CTAAATAAGCTGAAACGAGAAAG (D) GCTTGACACAGTGTTTGGATCCATT; CTTTCTCGTTTCAGCTTATTTAG 

39. Which of the following techniques is used to identify protein-protein interactions? (A) Northern blot analysis (B) Southern blot analysis (C) Western blot analysis (D) Yeast-two-hybrid system

40. Which of the following enzymes is used to generate complimentary DNA from RNA? (A) Klenow fragment (B) Reverse transcriptase (C) Taq polymerase (D) T4 DNA polymerase

41. Ubiquitin is a molecule involved in which of the following cellular processes? (A) Protein modification (B) RNA splicing (C) DNA degradation (D) Lipid synthesis

42. Elongation of telomeres by the enzyme telomerase and the processing of pre-mRNA to mRNA by the spliceosome share which of the following characteristics? (A) Both processes require double-stranded DNA for enzyme function. (B) Both processes require an RNA component for enzyme function. (C) Both processes require protease activity. (D) Both processes occur primarily in cytoplasm.

43. If cycloheximide is added to the cells, then (A) the DNA synthesis will be blocked. (B) the RNA synthesis will be blocked. (C) the protein synthesis will be blocked. (D) the ATP synthesis will be blocked.

44. Which of the following statements most accurately describe the glycosylation of a protein? (A) Glycosylation decreases the thermal stability of a protein (B) Glycosylation increases the thermal stability of a protein (C) Glycosylation decreases the proteolytic cleavage of a protein (D) Glycosylation increases the proteolytic cleavage of a protein

45. Which of the following statements is   TRUE   regarding the stacking gel used in SDS-PAGE? (A) It has a high pH relative to that of the resolving gel (B) It is usually located at the bottom of the resolving gel (C) It has a lower polyacrylamide concentration than that of the resolving gel (D) Its pH value is normally pH8.8.

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