11. Which of the following molecule is responsible for guiding RNA polymerase to initiate gene transcription in prokaryotes?
(A) Sigma factor
(B) Dna A
(C) TBP
(D) Rho
(E) None of the above

12. Which of the following statement is incorrect for bacteria two-component regulatory system? (A) Controlled by transferring phosphates between different components. (B) A protein with a DNA binding domain that can change its own structure. (C) A sensor kinase is involved. (D) Autophosphorylation on the DNA binding protein may change its DNA- binding ability. (E) Two-component regulatory system is found in many bacteria, but also in lower eukaryotes.

13. DNA sequence that are rich in guanine and cytosine are called ______ and are usually targets of _____.(A) interins; acetylation (B) promoters; transcriptional regulation (C) enhancers; mediator binding (D) GC islands; methylation (E)GC islands; acetylation

14. Which of the following mechanism does not explain the diverse gene products generated from a single gene? (A) Protein splicing (B) RNA polyadenylation (C) Alternative promoter selection (D) RNA base modification (E) mRNA alternative splicing

15. Which of the following feature do you expect NOT to see in heterochromatin? (A) Massive DNA methylation (B) The presence of histone deacetylase (C) The histone acetylase (D) The methylcytosine binding proteins (E) None of the above

16. Which of the following is the primase that synthesizes primers during DNA replication in E. coli? (A) DnaG (B) DnaA (C) HU protein (D) DnaB (E) RNA polymerase

17. Which of the following subunits of the DNA polymerase III holoenzyme is referred to as the "sliding clamp"? (A) α (B) β (C) γ (D) δ (E) θ

18. Put the following steps of E. coli primosome assembly in the correct order. (1) DnaG binds. (2) DnaA binds to oriC at dnaA boxes. (3) DnaB binds to the open complex. (4) DnaA, ATP, and HU protein melt the DNA. (A) 4, 1, 2, 3 (B) 3, 4, 1, 2 (C) 4, 2, 1, 3 (D) 2, 4, 3, 1 (E) 2, 4, 1, 3

19. Primer extension is used to _____ (A) locate the binding of RNA polymerase (B) determine the interaction of RNA and proteins (C) determine the transcriptional start site (D) generate primers for PCR (E) none of the above

20. Which of the following is NOT true concerning immunoglobulin gene recombination signal sequences? (A) There are conserved heptamers. (B) There are conserved nonamers. (C) Recombination occurs between a 12 bp signal and a 23 bp signal. (D) Recombination only occurs between two heptamers. (E) The conserved sequences are separated by a nonconserved sequence of either a 12 bp or a 23 bp sequence.

51媽厝國小位於溪湖鎮(A)中華路(B)民權路(C)湳底路。

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