32. Which of the following is   NOT   the possible action for miRNA?
(A) translation initiation blockade
(B) translation elongation blockade
(C) mRNA degradation
(D) stimulate translation
(E) targets viral DNAs

33. Which of the following sequence is responsible for the protein translation initiation in most eukaryotic mRNA transcripts? (A) Kozak sequence (B) Shine-Dalgarno sequence (C) TATA Box sequence (D) Internal Ribosome Entry sequence (E) PolyA sequence

34. Which of the following sequence is responsible for the eukaryotic transcription initiation? (A) Kozak sequence (B) Shine-Dalgarno sequence (C) TATA Box sequence (D) Internal Ribosome Entry sequence (E) PolyA sequence

35. Which of the following factor is   NOT   a GTP binding protein involved in protein translation? (A) IF-2 (B) IF-3 (C) EF-Tu (D) EF-G (E) RF3

36. Which of the following factor is necessary for the translocation step in protein translation in cells? (A) IF-2 (B) IF-3 (C) EF-Tu (D) EF-G (E) RF3 

37. Following the above description, if this mixture is applied to a column of DEAE- Sephadex at pH 7.0, which proteins will bind to the column? (A) All (B) 3, 4 (C) 1, 4 (D) 1, 3 (E) 1, 2

38. Following the above description, if this mixture is applied to a column of DEAE- Sephadex at pH 8.0, which proteins will bind to the column? (A) All (B) 3, 4 (C) 1, 4 (D) 1, 3 (E) 1, 2

39. Following the above description, if you first use Sephadex G-100 gel filtration column to do the purification, what is the elution order for the four proteins (＞, begin with the faster one； =, equal)? (A) 3 = 4 ＞ 1 = 2 (B) 2 = 3 ＞ 1 = 4 (C) 2 = 4 ＞ 1 = 3 (D) 1 = 4 ＞ 2 = 3 (E) 1 = 2 ＞ 3 = 4

40. Quantitative real-time PCR (qPCR) can be used to quantify mRNA levels of genes in different samples. The cycle numbers for the measurement of mRNA expression of gene X in sample A and B are 25 and 30, respectively, and those of GAPDH (a house keeping gene) in sample A and B are 12 and 13, respectively. What is the fold-difference in gene X expression between sample A and B? The PCR efficiencies for gene X and GAPDH are closed to 100%. (A) A is 4-folds higher than B. (B) A is 16-folds higher than B. (C) B is 4-folds higher than A. (D) B is 16-folds higher than A. (E) None of the above

1. According to the quantum mechanics, which is the correct statement for 3s, 3p and 3d of a helium ion, He⁺? (A) The orbital radii are the same for 3s, 3p and 3d. (B) 3s, 3p and 3d are degenerate. (C) The order of their energy levels is 3s ＜ 3p ＜ 3d. (D) 3s, 3p and 3d have the same number of the radial nodes. (E) The order of their energy levels is 3s ＞ 3p ＞ 3d.

2. What is the best description for the curved line in the figure? Please note that a₀ is the Bohr radius. (A) the radial distribution function of 2s of a hydrogen atom. (B) the radial distribution function of 3s of a hydrogen atom. (C) the radial distribution function of 3p of a hydrogen atom. (D) the radial wavefunction of 2s of a hydrogen atom. (E) the radial wavefunction of 3p of a hydrogen atom.

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