6. Which of the following evidence can be considered as supports or necessity for discontinuous synthesis of one strand of double-stranded DNA molecules?

(A) the presence of Okazaki fragments as replication intermediates

(B) DNA polymerase requires a primer

(C) DNA replicates unidirectionally

(D) DNA replicates bidirectionally

7. Which one concerning hybrid dysgenesis in Drosophila is true?(A) Hybrid dysgenesis occurs when the P element is contributed by the egg and sperm is devoid of P element.(B) Hybrid dysgenesis occurs when both parents (egg and sperm donors) carry P element.(C) Transposition of P element is responsible for killing somatic cells of F1 hybrid(D) The molecular basis of hybrid dysgenesis lies at the tissue-specific splicing of the primary transcript of transposase

8. Which one of the following processes requires RecA activity?(A) activating immunoglobulin gene rearrangement(B) resolving a Holliday junction during recombination(C) activating SOS gene expression(D) Integration of a phage genome to bacterial chromosome

9. Which of the following enzymes uses RNA to synthesize DNA?(A) Klenow enzyme(B) RNA replicase(C) telomerase(D) Escherichia coli DNA polymerase IV

10. Which statement is not true for class I and III promoters in the eukaryotes?(A) For eukaryotic RNA polymerase III-dependent promoters (class III promoter), the TATA box is recognized by the general transcription factor TFIIIB.(B) For class I promoter in human, the core-binding factor SL1 is required to recruit RNA polymerase I.(C) TFIIIB acts as an assembly factor that binds to the internal promoter and helps TFIIIA to bind to a region just upstream of the transcription start site.(D) TFIIIA is required for transcription of the 5S rRNA genes, but not for the tRNA genes.(E) Although class I promoters are variable in sequence across species, they do contain the AT-rich initiator with a conserved sequence.

11. Please choose one wrong statement for the roles of different general transcription factors in eukaryotic RNA polymerases.(A) TATA-binding factor (TBP) is also required for transcription in the Archaea.(B) The TBP-associated factor 1 (TAF1) serves as an assembly factor around which other TAFs can aggregate and also contains a histone acetyltransferase activity.(C) TFIIB is a general transcription factor with its C-terminal domain to interact with RNA polymerase II and can determine the direction of transcription.(D) TBP-free TAF-containing complex (TFTC) can sponsor the formation of preinitiation complex of RNA polymerase II without the presence of TFIID.(E) The negative cofactor 2 (NC2) can stimulate RNA polymerase II-related gene transcription from downstream promoter element (DPE)-containing promoters.

12. Which of the following statements is true for gene transcription in the eukaryotes?(A) Tfg2 is the second largest subunit of TFIIF and is homologous to bacterial alpha-subunit of RNA polymerase.(B) Mediator is a collection of proteins that is a part of most RNA polymerase II preinitiation complexes and is not required for transcription initiation, but is required for activated transcription.(C) TFIIIA is a general transcription factor for RNA polymerase III and contains 9 zinc finger structures in which one finger containing 6 cysteines to coordinate 2 zinc ions.(D) In the absence of ligand, the thyroid hormone receptor exists as an inactive form in cytoplasm complexed with heat shock protein 90.(E) Silencers are DNA-binding proteins that can cause strong bending in DNA.

13. Please choose one of the following techniques that cannot be used to quantify the level of gene expression.(A) Nuclear run-off assay.(B) Northern blot analysis.(C) S1 mapping.(D) RFLP analysis.(E) RNase protection.

14. Choose one wrong statement from the followings regarding eukaryotic transcription?(A) In flowing plants, RNA polymerase IV and V are involved in gene silencing, which are performed by RNA polymerase II in other eukaryotes.(B) TFIIA can interfere with the interaction between TAF1 of RNA polymerase II N-terminal domain & DNA-binding surface of TBP, freeing up TATA-binding protein (TBP) for promoter binding.(C) Rpb10 and Rpb12 are common subunits in all three yeast polymerases.(D) An enhanceosome is a collection of DNA sequences (enhancers) that can enhance transcription by themselves.(E) The incoming nucleotide first encounters the E ("entry") site and the "A" site of RNA polymerase II is where a phosphodiester bond forms.

15. A DNA element found in prokaryotes that binds tightly to a specific repressor and thereby regulates the expression of adjoining genes is called ______.(A) Enhancer(B) Promoter(C) Terminator(D) Suppressor(E) Operator

16. Which of the following is a possible mechanism for the action of NusA in termination?(A) It binds to NusB to promote detachment of the RNA polymerase for the DNA template.(B) It interacts with ρ10 to promote dissociation of the RNA polymerase.(C) It binds with N to promote hairpin loop formation.(D) It stimulates termination at intrinsic terminator by facilitating hairpin loop formation.(E) It binds to the RNA polymerase causing it to stall.

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