複選題

25. Which of the following protein binds to LDL receptor?
(A) Apo Al
(B) Apo B-48
(C) Apo C-II
(D) Apo D
(E) Apo E

26. What are the products of phosphorylase and debranching enzyme? (A) glucose 6-phosphate and glucose 1-phosphate (B) glucose 1-phosphate and fructose 6-phosphate (C) glucose 1-phosphate and glucose (D) glucose 6-phosphate and UDPGlc (E) glucose 1-phosphate and 1→6 linked glycogen.

27. Which following reaction has a negative free energy physiologically? (A) Glucose 6-phosphate + ADP → Glucose + ATP (B) Fructose 1,6-bisphosphate + ADP → fructose 6-phosphate + ATP (C) 1,3-Bisphosglycerate + ADP → 3-phosphoglycerate + ATP (D) Phosphoenolpyruvate + ADP → pyruvate + ATP (E) Carbonyl phosphate + ADP → CO2 + ATP

28. Which two enzymes are located in mitochondria? (A) Carbamoyl phosphate synthase I and ornithine transcarbamoylase (B) Argininosuccinate synthase and argininosuccinase (C) Carbamoyl phosphate synthase I and argininosuccinate synthase (D) Argininosuccinase and arginase (E) Carbamoyl phosphate synthase I and arginase.

29. What enzyme is responsible for the conversion between serine and glycine? (A) Convertase (B) Serine hydroxylase (C) Glycine methyltransferase (D) Serine methylase (E) Serine hydroxymethyltransferase.

30. What amino acid catabolism needs α-keto acid decarboxylase? If the decarboxylase is defect, it will cause maple syrup urine disease. (A) alanine (B) valine (C) tryptophan (D) phenylalanine (E) tyrosine.

31. According to the table above, which of the following DNA sequences is the putative coding region of the peptide? (A) ATG CCT TAC GAT GTG CCT GAT TAT GCT (B) TAA CCC TAT GAT GTA CCC GAC TAC GCC (C) TAT CCA TAA GAC GTC CCA GAT TAT GCA (D) TAC CCG TAT GAC GTC CCG GAC TAC GCG (E) TAG CCG TAC GAT GTT CCT GAT TAT GCC

32. To isolate the full-length gene, Wen-Liang will design synthetic DNA probes with 18 nucleotides to screen cDNA libraries prepared from viral infected serum. According to the table above, which of the following peptides will minimize the number of DNA probes for the screening? (A) YPYDVP (B) PYDVPD (C) YDVPDY (D) DVPDYA (E) All are the same

33. When he designs the PCR primer pairs that contain the restriction enzyme cutting sequences, which of the following primer pairs is his best choice? (A) the forward primer with BamH I; the reverse primer with Xho I (B) the forward primer with Xho I; the reverse primer with EcoR I (C) the forward primer with BamH I; the reverse primer with Hind III (D) the forward primer with EcoR I; the reverse primer with Xho I (E) the forward primer with Hind III; the reverse primer with BamH I

34. When Wen-Liang transfects the subcloned plasmid into the HEK 293T cell line and checks the gene expression by running the SDS-PAGE, what is the protein size he will expect to observe on the gel? (A) 293 ka (B) 64 ka (C) 1734 ka (D) 578 ka (E) 193 ka

35. To further confirm the gene expression, Wen-Liang can use the following methods EXCEPT (A) Southern blot (B) Northern blot (C) Western blot (D) Quantitative real-time PCR (E) None of them

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