24. Which of the following enzymes can digest dsRNA and play a role in RNA
interference?
(A) Dicer;
(B) RNasin;
(C) Argonaute;
(D) RNase A;
(E) RNase P

25. Which of the following methods would you use to detect DNA and protein interaction in vivo? (A) Electrophoretic mobility shift assay; (B) Chromatin Immunoprecipitation; (C) Yeast two-hybrid assay; (D) Real time PCR; (E) DNA sequencing.

26. Which of the following descriptions is not a function of the poly A tail of mRNAs? (A) protects the mRNA from degradation; (B) enhances translatability of the mRNA; (C) enhances transport of the mRNA to the cytoplasm; (D) enhances splicing of the mRNA; (E) prevent expression of the mRNA.

27. Which of the following descriptions is not true for Agrobacterium-mediated transformation gene transformation. (A) It is a particularly useful in plants; (B) Also called gene gun; (C) Using a T-DNA plasmid to introduce a gene into plants; (D) It is a natural genetic engineer. (E) Also can transfer DNA into non-plant cells

28. Spliceosome contains several snRNPs. Which of the following description of each snRNP is not matched with its function? (A) U1: base pairs with 5' splice site of pre-mRNA; (B) U2: base pairs with the conserved sequence at splicing branch point of pre-mRNA; (C) U3: base pairs with 3' splice site of pre-mRNA; (D) U4: Base pairs with U6; (E) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon.

29. Which is correct order of following stages in transcription initiation?(1) Forming the open promoter complex.(2) Forming the closed promoter complex.(3) Promoter clearance.(4) Incorporating first few nucleotides.(A) 1, 2, 3, 4; (B) 1, 4, 3, 2; (C) 2, 1, 4, 3; (D) 1, 2, 4, 3; (E) 3, 1, 2, 4.

30. Multiple forms of eukaryotic RNA polymerase. Which of the followingdescription about the roles of eukaryotic RNA polymerases is not correct?(A) RNA polymerase I synthesize 18S rRNA; (B) RNA polymerase II synthesizemRNAs; (C) RNA polymerase synthesize tRNAs; (D) RNA polymerase IV synthesize　5S rRNA;(E) RNA-dependent RNA polymerase catalyzes the replication of RNA froman RNA template.

1. Biochemical and genetic experiments have demonstrated that the______ of tRNA are important for recognition by its cognate aminotransferase-tRNA synthetase.(A) acceptor stem and anticodon loop(B) T loop and variable loop(C) anticodon loop and T loop(D) variable loop and D loop(E) D loop and T loop

2. Which of the following molecules does not resemble a tRNA molecule?(A) tmRNA(B) EF-Tu(C) puromycin(D) RRF(E) suppressor tRNA

3. Which of the following antibiotics does not inhibit protein synthesis by binding to the ribosome?(A) chloroamphenicol(B) streptomycin(C) ampicillin(D) erythromycin(E) tetracycline

4. Put the following steps of prokaryotic translation initiation in the correct order.(1) Binding of IF1, IF2, and GTP to the 30S subunit.(2) Binding of IF3 to the 30S subunit.(3) Binding of the 50S subunit and loss of IF1 and IF3.(4) Dissociation of the 70S ribosome.(5) Formation of the 70S initiation complex by dissociation of IF2 and GTP hydrolysis.(6) Formation of the 30S initiation complex.(A) 1,2,3,4,5,6(B) 2,1,6,3,5,4(C) 5,6,3,2,1,4(D) 4,2,1,6,3,5(E) 2,4,1,6,3,5

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