27. Which of the following splicing factors base-pair with pre-mRNA?
(A) U6
(B) U5
(C) U4
(D) U2
(E) U1

28. The synthesis of mRNA's that encode the proteins of eukaryotic RNA polymerase occurs in the: (A) cytoplasm (B) nuclear envelope (C) nucleolus (D) euchromatin (E) heterochromatin

複選題29. Which of the following description of RNA processing events is correct? (A) Cap is a modified 7-methylguanosine that 5'-3' links to the 5'terminus of RNA. (B) RNA polyadenylation only happens in nucleus. (C) RNA cap can be labeled with [B,y-32P]ATP but not with [y-32P]ΑΤΡ. (D) RNA polymerase II transcription of a gene usually stops at the polyadenylation site. (E) Once an mRNA loses its poly（A） tail, it is imported back into the nucleus for replenishing poly（A）tail.

30. Which of the following snRNP is mismatched with its function? (A) U1: base pairs with 5' splice site of mRNA. (B) U2: base pairs with the conserved sequence at splicing branch point. (C) U4: base pairs with 3' splice site of mRNA. (D) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon. (E) U6: base pairs with 5' end of the intron.

複選題31. The functions of the mRNA cap include: (A) protects the mRNA from degradation (B) enhances the translatability of the mRNA (C) enhances transport of the mRNA to the cytoplasm (D) enhances splicing out the first intron of the mRNA (E) aids transcription termination

32. Which of the following is   NOT   the possible action for miRNA? (A) translation initiation blockade (B) translation elongation blockade (C) mRNA degradation (D) stimulate translation (E) targets viral DNAs

33. Which of the following sequence is responsible for the protein translation initiation in most eukaryotic mRNA transcripts? (A) Kozak sequence (B) Shine-Dalgarno sequence (C) TATA Box sequence (D) Internal Ribosome Entry sequence (E) PolyA sequence

34. Which of the following sequence is responsible for the eukaryotic transcription initiation? (A) Kozak sequence (B) Shine-Dalgarno sequence (C) TATA Box sequence (D) Internal Ribosome Entry sequence (E) PolyA sequence

35. Which of the following factor is   NOT   a GTP binding protein involved in protein translation? (A) IF-2 (B) IF-3 (C) EF-Tu (D) EF-G (E) RF3

36. Which of the following factor is necessary for the translocation step in protein translation in cells? (A) IF-2 (B) IF-3 (C) EF-Tu (D) EF-G (E) RF3 

37. Following the above description, if this mixture is applied to a column of DEAE- Sephadex at pH 7.0, which proteins will bind to the column? (A) All (B) 3, 4 (C) 1, 4 (D) 1, 3 (E) 1, 2

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