18. The consensus sequence of splicing signal at the first two bases and the last two bases of pre-mRNA are
(A) GG-AG;
(B) GU-AG;
(C) CU-CG;
(D) GU-AU;
(E)GA-CG.

19. Which of the following statements is not true about the TATA-box-binding protein (TBP)? (A) TFIID contains TBP and TBP-associated factors; (B) Different TBP have been identified from yeast, fruit fly, plant, and human, and they are more than 80% identical in amino acid sequence; (C) TBP binds in the minor, rather than the major, groove of DNA; (D) The TBP functions not only in transcription of class II genes, but also in transcription of class I and class III genes; (E) TBP has two known enzymatic activities, Histon acetyltransferase (HAT) and Kinase.

20. If a transposable element can transpose on its own because it contains (A) transposase; (B) DNA repeats; (C) origin of replication; (D) antibiotic resistance; (E) Tn10.

21. Which is correct order of following steps in translation elongation? (1) EF-G, with GTP, translocates peptidyl-tRNA to the P site. (2) EF-Tu, with GTP, binds an aminoacyl-tRNA to the ribosomal A site. (3) Peptidyl transferase forms a peptide bond between the peptide in the P site and the newly arrived aminoacyl-tRNA in the A site. (A) 3, 1, 2; (B) 3, 2, 1; (C) 1, 2, 3; (D) 1, 3, 2; (E) 2, 3, 1.

22. Which of following proteins are not need in DNA replication fork during DNA replication? (A) Helicase; (B) Single-strand DNA-binding proteins; (C) Topoisomerase; (D) DNA polymerase; (E) recombinase.

23. All of the following agents causes DNA damage (mutagen) except (A) ampicillin; (B) deaminating agents; (C) sodium nitrite; (D) ethylmethanesulfonate; (E) ethidium bromide.

24. Which of the following enzymes can digest dsRNA and play a role in RNAinterference?(A) Dicer; (B) RNasin; (C) Argonaute; (D) RNase A; (E) RNase P

25. Which of the following methods would you use to detect DNA and protein interaction in vivo? (A) Electrophoretic mobility shift assay; (B) Chromatin Immunoprecipitation; (C) Yeast two-hybrid assay; (D) Real time PCR; (E) DNA sequencing.

26. Which of the following descriptions is not a function of the poly A tail of mRNAs? (A) protects the mRNA from degradation; (B) enhances translatability of the mRNA; (C) enhances transport of the mRNA to the cytoplasm; (D) enhances splicing of the mRNA; (E) prevent expression of the mRNA.

27. Which of the following descriptions is not true for Agrobacterium-mediated transformation gene transformation. (A) It is a particularly useful in plants; (B) Also called gene gun; (C) Using a T-DNA plasmid to introduce a gene into plants; (D) It is a natural genetic engineer. (E) Also can transfer DNA into non-plant cells

28. Spliceosome contains several snRNPs. Which of the following description of each snRNP is not matched with its function? (A) U1: base pairs with 5' splice site of pre-mRNA; (B) U2: base pairs with the conserved sequence at splicing branch point of pre-mRNA; (C) U3: base pairs with 3' splice site of pre-mRNA; (D) U4: Base pairs with U6; (E) U5: associates with last nucleotide in one exon and the first nucleotide in the next exon.

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