5. How is trypsinogen converted to trypsin?
(A) A protein kinase-catalyzed phosphorylation converts trypsinogen to trypsin
(B) An increase in Ca²⁺ concentration promotes the conversion
(C) Proteolysis of trypsinogen forms trypsin
(D) Trypsinogen dimers bind an allosteric modulator, cAMP, causing dissociation into active trypsin monomers
(E) Two inactive trypsinogen dimers pair to form an active trypsin tetramer.

6. Which of the following is not a reducing sugar? (A) Fructose (B) Glucose (C) Glyceraldehyde (D) Ribose (E) Sucrose.

7. In DNA sequencing by the Sanger (dideoxy) method (A) radioactive dideoxy ATP is included in each of four reaction mixtures before enzymatic synthesis of complementary strands (B) specific enzymes are used to cut the newly synthesized DNA into small pieces, which are then separated by electrophoresis (C) the dideoxynucleotides must be present at high levels to obtain long stretches of DNA sequence (D) the role of the dideoxy CTP is to occasionally terminate enzymatic synthesis of DNA where Gs occur in the template strands (E) the template DNA strand is radioactive

8. Which of the following contains an ether-linked alkyl group? (A) Cerebrosides (B) Gangliosides (C) Phosphatidyl serine (D) Platelet-activating factor (E) Sphingomyelin

9. A compound containing N-acetylneuraminic acid (sialic acid) is (A) cardiolipin (B) ganglioside GM2 (C) phosphatidylcholine (D) platelet-activating factor (E) sphingomyelin

10. Which of these statements about the composition of biological membranes is false? (A) In a given eukaryotic cell type (for example, a hepatocyte), all intracellular membranes have essentially the same complement of lipids and proteins (B) The carbohydrate found in membranes is virtually all part of either glycolipids or glycoproteins (C) The plasma membranes of the cells of vertebrate animals contain more cholesterol than the mitochondrial membranes (D) The ratio of lipid to protein varies widely among cell types in a single organism (E) Triacylglycerols are not commonly found in membranes

11. Ubiquitin is a (A) component of the electron transport system (B) protease (C) protein kinase (D) protein phosphorylase (E) protein that tags another protein for proteolysis

12. Which of the following does not involve cyclic AMP? (A) Regulation of glycogen synthesis and breakdown (B) Regulation of glycolysis (C) Signaling by acetylcholine (D) Signaling by epinephrine (E) Signaling by glucagon

13. During strenuous exercise, the NADH formed in the glyceraldehyde 3-phosphate dehydrogenase reaction in skeletal muscle must be reoxidized to NAD+ if glycolysis is to continue. The most important reaction involved in the reoxidation of NADH is (A) dihydroxyacetone phosphate  glycerol 3-phosphate (B) glucose 6-phosphate  fructose 6-phosphate (C) isocitrate  -ketoglutarate (D) oxaloacetate  malate (E) pyruvate  lactate

14. An enzyme used in both glycolysis and gluconeogenesis is (A) 3-phosphoglycerate kinase (B) glucose 6-phosphatase (C) hexokinase (D) phosphofructokinase-1 (E) pyruvate kinase

15. The oxidation of 3 mol of glucose by the pentose phosphate pathway may result in the production of (A) 2 mol of pentose, 4 mol of NADPH, and 8 mol of CO2 (B) 3 mol of pentose, 4 mol of NADPH, and 3 mol of CO2 (C) 3 mol of pentose, 6 mol of NADPH, and 3 mol of CO2 (D) 4 mol of pentose, 3 mol of NADPH, and 3 mol of CO2 (E) 4 mol of pentose, 6 mol of NADPH, and 6 mol of CO2

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